OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		- Due to light-induced auto-oxidation, the stock DCF-DiOxyQ solution and all subsequent DCF-DiOxy and DCFH solutions must be protected from light.

Downstream tips
- Due to light-induced auto-oxidation, the stock DCF-DiOxyQ solution and all subsequent DCF-DiOxy and DCFH solutions must be protected from light.

Publication protocol

Plasma ROS/RNS was determined by using the fluorescence kit STA-347 (Cell Biolabs Inc., San Diego, CA, USA) at excitation and emission wavelengths of 480 and 530 nm, respectively, in a SpectraMax Gemini XS Fluorometer. MDA was assessed by using the NWLSS™ assay (Vancouver, WA, Canada).

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Manufacturer protocol

Download the product protocol from Cell Biolabs for OxiSelect™ In Vitro ROS/RNS Assay Kit (Green Fluorescence) below.

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