STEAP2 metalloreductase

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	PC3 cells purchased from American Type Culture Collection (ATCC, LGC Standard) were maintained in RPMI 1640 media (Gibco, Life Technologies, UK) supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, and 100 U/ml penicillin/streptomycin (Gibco, Life Technologies, UK) at 37 °C and 5% CO2. STEAP2 expression was reduced using siRNA S48991, a non-specific siRNA (4390843) was used as a control (Life Technologies, UK) and Lipofectamine RNAiMAX (Life Technologies, UK) was used according to manufacturer’s instructions. PC3 cells were incubated with the siRNA complex for 72 h.

Protocol tips

PC3 cells purchased from American Type Culture Collection (ATCC, LGC Standard) were maintained in RPMI 1640 media (Gibco, Life Technologies, UK) supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, and 100 U/ml penicillin/streptomycin (Gibco, Life Technologies, UK) at 37 °C and 5% CO2. STEAP2 expression was reduced using siRNA S48991, a non-specific siRNA (4390843) was used as a control (Life Technologies, UK) and Lipofectamine RNAiMAX (Life Technologies, UK) was used according to manufacturer’s instructions. PC3 cells were incubated with the siRNA complex for 72 h.

Publication protocol

PC3 cells purchased from American Type Culture Collection (ATCC, LGC Standard) were maintained in RPMI 1640 media (Gibco, Life Technologies, UK) supplemented with 10% fetal bovine serum (FBS), 2 mM glutamine, and 100 U/ml penicillin/streptomycin (Gibco, Life Technologies, UK) at 37 °C and 5% CO2. STEAP2 expression was reduced using siRNA S48991, a non-specific siRNA (4390843) was used as a control (Life Technologies, UK) and Lipofectamine RNAiMAX (Life Technologies, UK) was used according to manufacturer’s instructions. PC3 cells were incubated with the siRNA complex for 72 h.

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