DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit

ROS assay cell type - rat kidney and pancreas tissue

Experiment
ROS assay cell type - rat kidney and pancreas tissue
Product
DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit from Abcam
Manufacturer
Abcam

Protocol tips

Upstream tips
- Dilute compounds of interest in complete media without phenol red.
Protocol tips
- Incubate DCFDA and compounds for the desired period of time (30 – 45 minutes).

- 1 hour prior to completion of the treatment, dilute DCFDA at 2X of the final concentration desired in the same media used for treatment

Publication protocol

ROS production in ZDF and ZL rat kidney and pancreas was measured using the DCFDA fluorescence method as described before [16].

Full paper   Login or join for free to view the full paper.

Reviews

DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit from Abcam has not yet been reviewed for this experiment

We'd love it if you would be the first to write a review!

Discussion

Start your discussion

Share your thoughts or question with experts in your field

Start a discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing ROS assay cell type - rat kidney and pancreas tissue using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit from Abcam.

Paper title
Increased metabolic stress in Zucker diabetic fatty rat kidney and pancreas.
Full paper
Login or join for free to view the full paper.

Manufacturer protocol

Download the product protocol from Abcam for DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit below.

Download PDF Download manufacturer protocol

Videos

Check out videos that might be relevant for performing ROS assay cell type - rat kidney and pancreas tissue using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit from Abcam. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.

We haven't found any additional videos for this experiment / product combination yet.

Become shareholder Discussions About us Contact Privacy Terms