DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit

Protocol tips

Upstream tips	Protocol tips	Downstream tips
- Dilute compounds of interest in complete media without phenol red.	- Incubate DCFDA and compounds for the desired period of time (30 – 45 minutes). - 1 hour prior to completion of the treatment, dilute DCFDA at 2X of the final concentration desired in the same media used for treatment

Upstream tips
- Dilute compounds of interest in complete media without phenol red.
Protocol tips
- Incubate DCFDA and compounds for the desired period of time (30 – 45 minutes). - 1 hour prior to completion of the treatment, dilute DCFDA at 2X of the final concentration desired in the same media used for treatment

Publication protocol

ROS production in ZDF and ZL rat kidney and pancreas was measured using the DCFDA fluorescence method as described before [16].

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Manufacturer protocol

Download the product protocol from Abcam for DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit below.

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