Gibco™Glasgow's MEM (GMEM)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	cortex differentiation medium (9,000 cells per well) containing Glasgow-MEM, 20% Knockout Serum Replacement, and 20 μM Y-27632. IWR1e (Wnt inhibitor) and SB431542 (TGFβ inhibitor) were added to culture to reach 3 and 5 μM, respectively, from day 0 to day 18

Protocol tips
cortex differentiation medium (9,000 cells per well) containing Glasgow-MEM, 20% Knockout Serum Replacement, and 20 μM Y-27632. IWR1e (Wnt inhibitor) and SB431542 (TGFβ inhibitor) were added to culture to reach 3 and 5 μM, respectively, from day 0 to day 18

Publication protocol

cortex differentiation medium (9,000 cells per well) containing Glasgow-MEM, 20% Knockout Serum Replacement, and 20 μM Y-27632. IWR1e (Wnt inhibitor) and SB431542 (TGFβ inhibitor) were added to culture to reach 3 and 5 μM, respectively, from day 0 to day 18

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Stem cell Differentiation media hESCs differentiation into cortical neuroepithelium (NE) using Gibco™Glasgow's MEM (GMEM) from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™Glasgow's MEM (GMEM) below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Stem cell Differentiation media hESCs differentiation into cortical neuroepithelium (NE) using Gibco™Glasgow's MEM (GMEM) from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.