Gibco™DMEM/F-12

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	3N medium consisted of a 1:1 mixture of N2- and B27-containing media. N2 medium: DMEM/F12, N2 (GIBCO), 5 μg/ml Insulin, 1mM L-Glutamine, 100 μm non-essential amino acids, 100 μM 2-mercaptoethanol, 50 U/ml Penicillin and 50 mg/ml Streptomycin; B27 medium: Neurobasal (Invitrogen), B27 with or without vitamin A (GIBCO), 200 mM Glutamine, 50 U/ml Penicillin and 50 mg/ml Streptomycin. 3N medium was supplemented with either 1 μm Dorsomorphin (Tocris) or 500 ng/ml mouse Noggin-CF chimera (R&D Systems), and 10 μm SB431542 (Tocris) to inhibit TGFβ signaling during neural induction

Protocol tips

3N medium consisted of a 1:1 mixture of N2- and B27-containing media. N2 medium: DMEM/F12, N2 (GIBCO), 5 μg/ml Insulin, 1mM L-Glutamine, 100 μm non-essential amino acids, 100 μM 2-mercaptoethanol, 50 U/ml Penicillin and 50 mg/ml Streptomycin; B27 medium: Neurobasal (Invitrogen), B27 with or without vitamin A (GIBCO), 200 mM Glutamine, 50 U/ml Penicillin and 50 mg/ml Streptomycin. 3N medium was supplemented with either 1 μm Dorsomorphin (Tocris) or 500 ng/ml mouse Noggin-CF chimera (R&D Systems), and 10 μm SB431542 (Tocris) to inhibit TGFβ signaling during neural induction

Publication protocol

3N medium consisted of a 1:1 mixture of N2- and B27-containing media. N2 medium: DMEM/F12, N2 (GIBCO), 5 μg/ml Insulin, 1mM L-Glutamine, 100 μm non-essential amino acids, 100 μM 2-mercaptoethanol, 50 U/ml Penicillin and 50 mg/ml Streptomycin; B27 medium: Neurobasal (Invitrogen), B27 with or without vitamin A (GIBCO), 200 mM Glutamine, 50 U/ml Penicillin and 50 mg/ml Streptomycin. 3N medium was supplemented with either 1 μm Dorsomorphin (Tocris) or 500 ng/ml mouse Noggin-CF chimera (R&D Systems), and 10 μm SB431542 (Tocris) to inhibit TGFβ signaling during neural induction

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™DMEM/F-12 below.

Download manufacturer protocol

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