Gibco™ DMEM/F-12, GlutaMAX™ supplement

Protocol tips

Upstream tips	Protocol tips	Downstream tips
NMM Contains: DMEM/F12, Glutamax (500 ml, Life Technologies), Neurobasal medium (500 ml, Life Technologies), N2 supplement (1×, Life Technologies), B27 supplement (1×, Life Technologies), insulin (5 μg ml−1, Imgen), L-glutamine (1.5 mM, Life Technologies), non-essential amino acids (NEAA, 100 µM, Life Technologies), penicillin/streptomycin (100 U l−1, Life Technologies), beta-mercaptoethanol (10 µM, Life Technologies). Medium was stored at 4°C and used within 3 weeks.	Neural Maintenance Medium (NMM)+FGF2 (4 ng ml−1, Peprotech)+RI (10 µM), and distributed to anti-adhesive (Poly-HEMA, Sigma, P3932-10G) coated 6WPs in a 2 or 3:1 ratio (with 2.5 ml medium per well)

Upstream tips
NMM Contains: DMEM/F12, Glutamax (500 ml, Life Technologies), Neurobasal medium (500 ml, Life Technologies), N2 supplement (1×, Life Technologies), B27 supplement (1×, Life Technologies), insulin (5 μg ml−1, Imgen), L-glutamine (1.5 mM, Life Technologies), non-essential amino acids (NEAA, 100 µM, Life Technologies), penicillin/streptomycin (100 U l−1, Life Technologies), beta-mercaptoethanol (10 µM, Life Technologies). Medium was stored at 4°C and used within 3 weeks.
Protocol tips
Neural Maintenance Medium (NMM)+FGF2 (4 ng ml−1, Peprotech)+RI (10 µM), and distributed to anti-adhesive (Poly-HEMA, Sigma, P3932-10G) coated 6WPs in a 2 or 3:1 ratio (with 2.5 ml medium per well)

Publication protocol

Neural Maintenance Medium (NMM)+FGF2 (4 ng ml−1, Peprotech)+RI (10 µM), and distributed to anti-adhesive (Poly-HEMA, Sigma, P3932-10G) coated 6WPs in a 2 or 3:1 ratio (with 2.5 ml medium per well)

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™ DMEM/F-12, GlutaMAX™ supplement below.

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