Gibco™Ham's F-12 Nutrient Mix

Protocol tips

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	Iscove's modified Dulbecco's medium/Ham's F-12 1:1, chemically defined lipid concentrate, penicillin/streptomycin, monothioglycerol (450 μM), apo-transferrin (15 μg/ml) and crystallization-purified bovine serum albumin (5 mg/ml, Sigma). The medium was supplemented with insulin (1 μg/ml) and SB431542 (10 μM, Tocris) on day 0 (the starting day of differentiation culture).

Protocol tips
Iscove's modified Dulbecco's medium/Ham's F-12 1:1, chemically defined lipid concentrate, penicillin/streptomycin, monothioglycerol (450 μM), apo-transferrin (15 μg/ml) and crystallization-purified bovine serum albumin (5 mg/ml, Sigma). The medium was supplemented with insulin (1 μg/ml) and SB431542 (10 μM, Tocris) on day 0 (the starting day of differentiation culture).

Publication protocol

Iscove's modified Dulbecco's medium/Ham's F-12 1:1, chemically defined lipid concentrate, penicillin/streptomycin, monothioglycerol (450 μM), apo-transferrin (15 μg/ml) and crystallization-purified bovine serum albumin (5 mg/ml, Sigma). The medium was supplemented with insulin (1 μg/ml) and SB431542 (10 μM, Tocris) on day 0 (the starting day of differentiation culture).

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™Ham's F-12 Nutrient Mix below.

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