Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
human pluripotent stem cells were detached from the feeder layer by collagenase IV, dispersed into small clumps |
Iscove’s modified Dulbecco’s differentiation medium supplemented with 20% fetal calf serum, 1 mM l-glutamine, 0.1 mM beta-mercaptoethanol and 1% nonessential amino acid stock in ultra-low attachment plates (Corning) for 7 days. |
|
Upstream tips |
human pluripotent stem cells were detached from the feeder layer by collagenase IV, dispersed into small clumps |
Protocol tips |
Iscove’s modified Dulbecco’s differentiation medium supplemented with 20% fetal calf serum, 1 mM l-glutamine, 0.1 mM beta-mercaptoethanol and 1% nonessential amino acid stock in ultra-low attachment plates (Corning) for 7 days. |
Publication protocol
Iscove’s modified Dulbecco’s differentiation medium supplemented with 20% fetal calf serum, 1 mM l-glutamine, 0.1 mM beta-mercaptoethanol and 1% nonessential amino acid stock in ultra-low attachment plates (Corning) for 7 days.
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