Gibco™KnockOut™ DMEM

Protocol tips

Upstream tips	Protocol tips	Downstream tips
20 000 cells per 200 μl in a V-shaped well of a 96-well plate (Sumitomo Bakelite, Tokyo, Japan) with an ultra-low-cell adhesion surface	KnockOut DMEM (Life Technologies, Carlsbad, CA, USA) containing 15% KnockOut Serum Replacement (HyClone, Logan, UT, USA), 1% GlutaMAX (Life Technologies), 1% MEM Non-Essential Amino Acids (Life Technologies), 0.09% 2-mercaptoethanol (Life Technologies), and 0.1% Leukemia Inhibitory Factor (Wako, Osaka, Japan)

Upstream tips
20 000 cells per 200 μl in a V-shaped well of a 96-well plate (Sumitomo Bakelite, Tokyo, Japan) with an ultra-low-cell adhesion surface
Protocol tips
KnockOut DMEM (Life Technologies, Carlsbad, CA, USA) containing 15% KnockOut Serum Replacement (HyClone, Logan, UT, USA), 1% GlutaMAX (Life Technologies), 1% MEM Non-Essential Amino Acids (Life Technologies), 0.09% 2-mercaptoethanol (Life Technologies), and 0.1% Leukemia Inhibitory Factor (Wako, Osaka, Japan)

Publication protocol

KnockOut DMEM (Life Technologies, Carlsbad, CA, USA) containing 15% KnockOut Serum Replacement (HyClone, Logan, UT, USA), 1% GlutaMAX (Life Technologies), 1% MEM Non-Essential Amino Acids (Life Technologies), 0.09% 2-mercaptoethanol (Life Technologies), and 0.1% Leukemia Inhibitory Factor (Wako, Osaka, Japan)

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™KnockOut™ DMEM below.

Download manufacturer protocol

Videos

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