Gibco™DMEM/F-12

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Briefly, the iPS cells were stimulated with all-trans-retinoic acid (atRA) for 48 h, then atRA was removed. The iPS cells were cultured in fresh medium supplemented with E2, FSH and human growth hormone (hGH) for 48 h. The iPS cells were then cultured in fresh medium supplemented with anti-Müllerian hormone (AMH), estradiol, FSH and hGH for 48 h. Finally, the iPS cells were cultured in fresh medium supplemented with AMH, estradiol, inhibin α, inhibin β and transforming growth factor-β for 96 h.	Dulbecco's modified Eagle's medium:F12 (1:1) supplemented with 15% KnockOut™ Serum Replacement, 1 mM sodium pyruvate, 2 mM L-glutamine, 0.1 mM nonessential amino acids, 0.1 mM beta-mercaptoethanol, penicillin (25 U/ml)-streptomycin (925 mg/ml), Reconstituting Human Basic Fibroblast Growth Factor (15 ng/ml), Reconstituting Human Epidermal Growth Factor (15 ng/ml)

Upstream tips
Briefly, the iPS cells were stimulated with all-trans-retinoic acid (atRA) for 48 h, then atRA was removed. The iPS cells were cultured in fresh medium supplemented with E2, FSH and human growth hormone (hGH) for 48 h. The iPS cells were then cultured in fresh medium supplemented with anti-Müllerian hormone (AMH), estradiol, FSH and hGH for 48 h. Finally, the iPS cells were cultured in fresh medium supplemented with AMH, estradiol, inhibin α, inhibin β and transforming growth factor-β for 96 h.
Protocol tips
Dulbecco's modified Eagle's medium:F12 (1:1) supplemented with 15% KnockOut™ Serum Replacement, 1 mM sodium pyruvate, 2 mM L-glutamine, 0.1 mM nonessential amino acids, 0.1 mM beta-mercaptoethanol, penicillin (25 U/ml)-streptomycin (925 mg/ml), Reconstituting Human Basic Fibroblast Growth Factor (15 ng/ml), Reconstituting Human Epidermal Growth Factor (15 ng/ml)

Publication protocol

Dulbecco's modified Eagle's medium:F12 (1:1) supplemented with 15% KnockOut™ Serum Replacement, 1 mM sodium pyruvate, 2 mM L-glutamine, 0.1 mM nonessential amino acids, 0.1 mM beta-mercaptoethanol, penicillin (25 U/ml)-streptomycin (925 mg/ml), Reconstituting Human Basic Fibroblast Growth Factor (15 ng/ml), Reconstituting Human Epidermal Growth Factor (15 ng/ml)

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™DMEM/F-12 below.

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