Gibco™DMEM, low glucose, pyruvate

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Seeding cells in six-well plates (2 × 104 cells /well) with complete media reached 90% confluency. Dulbecco’s modified Eagle’s medium–low glucose (DMEM-LG, Life Technologies, USA) containing 10% fetal bovine serum (FBS, Life Technologies, USA).	medium plus 0.5 μM isobutyl xanthine (Sigma, MI, USA), 50 μM indomethacin (Sigma, MI, USA), 2.0 μM insulin (Sigma, MI, USA), 0.5 μM dexamethasone (Sigma, MI, USA), and 10% FBS

Upstream tips
Seeding cells in six-well plates (2 × 104 cells /well) with complete media reached 90% confluency. Dulbecco’s modified Eagle’s medium–low glucose (DMEM-LG, Life Technologies, USA) containing 10% fetal bovine serum (FBS, Life Technologies, USA).
Protocol tips
medium plus 0.5 μM isobutyl xanthine (Sigma, MI, USA), 50 μM indomethacin (Sigma, MI, USA), 2.0 μM insulin (Sigma, MI, USA), 0.5 μM dexamethasone (Sigma, MI, USA), and 10% FBS

Publication protocol

medium plus 0.5 μM isobutyl xanthine (Sigma, MI, USA), 50 μM indomethacin (Sigma, MI, USA), 2.0 μM insulin (Sigma, MI, USA), 0.5 μM dexamethasone (Sigma, MI, USA), and 10% FBS

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™DMEM, low glucose, pyruvate below.

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