Gibco™ DMEM, high glucose

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	DMEM high-glucose with L-Glutamine (Thermo Fisher Scientific, Dreieich, Germany), 10% fetal calf serum (Thermo Fisher Scientific, Dreieich, Germany), Penicillin/Streptomycin 100 mg/l (Merck, Darmstadt, Germany), Amphotericin B 2.5 mg/l (Merck, Darmstadt, Germany), dexamethasone 0.1 μM (Sigma Aldrich, Steinheim, Germany), ascorbic acid-2-phosphate 2.5 mg/l (Sigma Aldrich, Steinheim, Germany), beta glycerophosphate 10 mM (Merck, Darmstadt, Germany).

Protocol tips

DMEM high-glucose with L-Glutamine (Thermo Fisher Scientific, Dreieich, Germany), 10% fetal calf serum (Thermo Fisher Scientific, Dreieich, Germany), Penicillin/Streptomycin 100 mg/l (Merck, Darmstadt, Germany), Amphotericin B 2.5 mg/l (Merck, Darmstadt, Germany), dexamethasone 0.1 μM (Sigma Aldrich, Steinheim, Germany), ascorbic acid-2-phosphate 2.5 mg/l (Sigma Aldrich, Steinheim, Germany), beta glycerophosphate 10 mM (Merck, Darmstadt, Germany).

Publication protocol

DMEM high-glucose with L-Glutamine (Thermo Fisher Scientific, Dreieich, Germany), 10% fetal calf serum (Thermo Fisher Scientific, Dreieich, Germany), Penicillin/Streptomycin 100 mg/l (Merck, Darmstadt, Germany), Amphotericin B 2.5 mg/l (Merck, Darmstadt, Germany), dexamethasone 0.1 μM (Sigma Aldrich, Steinheim, Germany), ascorbic acid-2-phosphate 2.5 mg/l (Sigma Aldrich, Steinheim, Germany), beta glycerophosphate 10 mM (Merck, Darmstadt, Germany).

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Manufacturer protocol

Download the product protocol from Fisher Scientific for Gibco™ DMEM, high glucose below.

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