Dulbecco’s Modified Eagle’s Medium - high glucose

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	DMEM plus l-ascorbic acid-2-phosphate, pyruvic acid sodium salt (Sigma Aldrich), l-proline (Sigma Aldrich), ITS+ culture supplement (BD Bioscience, Erembodegem, Belgium) and TGF-β3 (R&D Systems, Abingdon, UK).

Protocol tips
DMEM plus l-ascorbic acid-2-phosphate, pyruvic acid sodium salt (Sigma Aldrich), l-proline (Sigma Aldrich), ITS+ culture supplement (BD Bioscience, Erembodegem, Belgium) and TGF-β3 (R&D Systems, Abingdon, UK).

Publication protocol

DMEM plus l-ascorbic acid-2-phosphate, pyruvic acid sodium salt (Sigma Aldrich), l-proline (Sigma Aldrich), ITS+ culture supplement (BD Bioscience, Erembodegem, Belgium) and TGF-β3 (R&D Systems, Abingdon, UK).

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Papers

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Dulbecco’s Modified Eagle’s Medium - high glucose below.

Download manufacturer protocol

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