Adipogenic Induction Medium

Protocol tips

Upstream tips	Protocol tips	Downstream tips
cultured with Mesenchymal Stem Cell Basal Medium (MSCBM, Lonza, Basel, Switzerland, #PT-3238) until the cells became 100% confluent.	adipogenic induction medium (Lonza, #PT-3102B) supplemented with h-insulin, L-glutamine, mesenchymal cell growth supplement (MCGS), dexamethasone, indomethacin, isobutylmethylxanthine (IBMX), and GA-1000 (Lonza, #PT-4135) for 4 days. Then, the cells were cultured in adipogenic maintenance medium (Lonza, #PT-3102A) supplemented with h-insulin, L-glutamine, MCGS, and GA-1000 (Lonza, #PT-4122)

Upstream tips
cultured with Mesenchymal Stem Cell Basal Medium (MSCBM, Lonza, Basel, Switzerland, #PT-3238) until the cells became 100% confluent.
Protocol tips
adipogenic induction medium (Lonza, #PT-3102B) supplemented with h-insulin, L-glutamine, mesenchymal cell growth supplement (MCGS), dexamethasone, indomethacin, isobutylmethylxanthine (IBMX), and GA-1000 (Lonza, #PT-4135) for 4 days. Then, the cells were cultured in adipogenic maintenance medium (Lonza, #PT-3102A) supplemented with h-insulin, L-glutamine, MCGS, and GA-1000 (Lonza, #PT-4122)

Publication protocol

adipogenic induction medium (Lonza, #PT-3102B) supplemented with h-insulin, L-glutamine, mesenchymal cell growth supplement (MCGS), dexamethasone, indomethacin, isobutylmethylxanthine (IBMX), and GA-1000 (Lonza, #PT-4135) for 4 days. Then, the cells were cultured in adipogenic maintenance medium (Lonza, #PT-3102A) supplemented with h-insulin, L-glutamine, MCGS, and GA-1000 (Lonza, #PT-4122)

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