Gibco™IMDM, powder

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Add 1μl/mL of CHIR99021 stock, 1μl/mL of KGF stock, 1μl/mL of FGF10 stock, 1μl/mL of BMP4 stock, 2μl/mL of EGF stock (optional) and 0.1μl/mL of Retinoic Acid stock to SFD+ medium to obtain a final concentration of 3 μM for CHIR99021, 10ng/ml for KGF, 10ng/ml for FGF10, 10ng/ml for BMP4, 20ng/ml for EGF (optional) and 50 nM for Retinoic Acid	750ml of reconstituted IMDM, 250ml of F-12, 7.5ml of BSA, 10ml of Glutamax, 5ml of N2, 10ml of B27 and 10ml of Penicillin/Streptomycin

Upstream tips
Add 1μl/mL of CHIR99021 stock, 1μl/mL of KGF stock, 1μl/mL of FGF10 stock, 1μl/mL of BMP4 stock, 2μl/mL of EGF stock (optional) and 0.1μl/mL of Retinoic Acid stock to SFD+ medium to obtain a final concentration of 3 μM for CHIR99021, 10ng/ml for KGF, 10ng/ml for FGF10, 10ng/ml for BMP4, 20ng/ml for EGF (optional) and 50 nM for Retinoic Acid
Protocol tips
750ml of reconstituted IMDM, 250ml of F-12, 7.5ml of BSA, 10ml of Glutamax, 5ml of N2, 10ml of B27 and 10ml of Penicillin/Streptomycin

Publication protocol

750ml of reconstituted IMDM, 250ml of F-12, 7.5ml of BSA, 10ml of Glutamax, 5ml of N2, 10ml of B27 and 10ml of Penicillin/Streptomycin

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™IMDM, powder below.

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