Gibco™Advanced DMEM/F-12

Protocol tips

Upstream tips	Protocol tips	Downstream tips
with NOG, SB, 500 ng/ml FGF4 (R&D Systems), and 2 µM CHIR99021 (Chiron, Stemgent)	Advanced DMEM/F12 plus N-2 and B27 supplement, 10 mM Hepes, 1× L-Glutamine (200 mM), 1× Penicillin-streptomycin (5000 U/ml, all from Life Technologies) with 200 ng/ml Noggin (NOG, R&D Systems) and 10 µM SB431542 (SB, Stemgent, Cambridge, MA)

Upstream tips
with NOG, SB, 500 ng/ml FGF4 (R&D Systems), and 2 µM CHIR99021 (Chiron, Stemgent)
Protocol tips
Advanced DMEM/F12 plus N-2 and B27 supplement, 10 mM Hepes, 1× L-Glutamine (200 mM), 1× Penicillin-streptomycin (5000 U/ml, all from Life Technologies) with 200 ng/ml Noggin (NOG, R&D Systems) and 10 µM SB431542 (SB, Stemgent, Cambridge, MA)

Publication protocol

Advanced DMEM/F12 plus N-2 and B27 supplement, 10 mM Hepes, 1× L-Glutamine (200 mM), 1× Penicillin-streptomycin (5000 U/ml, all from Life Technologies) with 200 ng/ml Noggin (NOG, R&D Systems) and 10 µM SB431542 (SB, Stemgent, Cambridge, MA)

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™Advanced DMEM/F-12 below.

Download manufacturer protocol

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