Gibco™DMEM/F-12, no glutamine

Protocol tips

Upstream tips	Protocol tips	Downstream tips
DMEM/F12 1% (vol/vol) FBS and 500 ng/mL of FGF10 to foregut basal medium	Add 1× N-2, 1× B27, 10 mM HEPES, 1× GlutaMAX and 1× pen–strep to advanced DMEM/F12 in a sterile hood. Store at 4 °C for up to 2 months. On the day of use, add 10 µM SB431542, 200 ng/mL Noggin, 1 µM SAG, 500 ng/mL FGF4 and 2 µM CHIR99021.

Upstream tips
DMEM/F12 1% (vol/vol) FBS and 500 ng/mL of FGF10 to foregut basal medium
Protocol tips
Add 1× N-2, 1× B27, 10 mM HEPES, 1× GlutaMAX and 1× pen–strep to advanced DMEM/F12 in a sterile hood. Store at 4 °C for up to 2 months. On the day of use, add 10 µM SB431542, 200 ng/mL Noggin, 1 µM SAG, 500 ng/mL FGF4 and 2 µM CHIR99021.

Publication protocol

Add 1× N-2, 1× B27, 10 mM HEPES, 1× GlutaMAX and 1× pen–strep to advanced DMEM/F12 in a sterile hood. Store at 4 °C for up to 2 months. On the day of use, add 10 µM SB431542, 200 ng/mL Noggin, 1 µM SAG, 500 ng/mL FGF4 and 2 µM CHIR99021.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™DMEM/F-12, no glutamine below.

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