Gibco™DMEM, low glucose, pyruvate

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Complete medium: LG-DMEM containing with 10% FBS	Complete medium supplemented with 10 ​ng/ml of TGF-β3 and 50 ​ng/ml of bone morphogenetic protein-2 (BMP-2) (R&D systems), 10−7 ​M dexamethasone, 40 ​μg/ml proline, 100 ​μg/ml pyruvate, 50 ​μg ascorbate-2-phosphate (all from Sigma-Aldrich), and 1:100 diluted insulin, transferrin, selenium (ITS) Universal Cell Culture Supplement Premix (Becton Dickson, Franklin Lakes, NJ)

Upstream tips
Complete medium: LG-DMEM containing with 10% FBS
Protocol tips
Complete medium supplemented with 10 ​ng/ml of TGF-β3 and 50 ​ng/ml of bone morphogenetic protein-2 (BMP-2) (R&D systems), 10−7 ​M dexamethasone, 40 ​μg/ml proline, 100 ​μg/ml pyruvate, 50 ​μg ascorbate-2-phosphate (all from Sigma-Aldrich), and 1:100 diluted insulin, transferrin, selenium (ITS) Universal Cell Culture Supplement Premix (Becton Dickson, Franklin Lakes, NJ)

Publication protocol

Complete medium supplemented with 10 ​ng/ml of TGF-β3 and 50 ​ng/ml of bone morphogenetic protein-2 (BMP-2) (R&D systems), 10−7 ​M dexamethasone, 40 ​μg/ml proline, 100 ​μg/ml pyruvate, 50 ​μg ascorbate-2-phosphate (all from Sigma-Aldrich), and 1:100 diluted insulin, transferrin, selenium (ITS) Universal Cell Culture Supplement Premix (Becton Dickson, Franklin Lakes, NJ)

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™DMEM, low glucose, pyruvate below.

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