Gibco™ DMEM, high glucose

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	HG-DMEM supplemented with 1% of FBS, 2 mM L-glutamine, 50 U/mL penicillin, 50 mg/mL streptomycin, 1 mM sodium pyruvate (all from Sigma-Aldrich), 1% ITS + 1 (1.0 mg/mL insulin from bovine pancreas, 0.55 mg/mL human transferrin, 0.5 μg/mL sodium selenite, 50 mg/mL bovine serum albumin and 470 μg/mL linoleic acid, Sigma-Aldrich), 0.1 mM dexamethasone, 0.1 mM L-ascorbic acid-2-phosphate, 10 ng/ml TGF-β1 (Peprotech)

Protocol tips

HG-DMEM supplemented with 1% of FBS, 2 mM L-glutamine, 50 U/mL penicillin, 50 mg/mL streptomycin, 1 mM sodium pyruvate (all from Sigma-Aldrich), 1% ITS + 1 (1.0 mg/mL insulin from bovine pancreas, 0.55 mg/mL human transferrin, 0.5 μg/mL sodium selenite, 50 mg/mL bovine serum albumin and 470 μg/mL linoleic acid, Sigma-Aldrich), 0.1 mM dexamethasone, 0.1 mM L-ascorbic acid-2-phosphate, 10 ng/ml TGF-β1 (Peprotech)

Publication protocol

HG-DMEM supplemented with 1% of FBS, 2 mM L-glutamine, 50 U/mL penicillin, 50 mg/mL streptomycin, 1 mM sodium pyruvate (all from Sigma-Aldrich), 1% ITS + 1 (1.0 mg/mL insulin from bovine pancreas, 0.55 mg/mL human transferrin, 0.5 μg/mL sodium selenite, 50 mg/mL bovine serum albumin and 470 μg/mL linoleic acid, Sigma-Aldrich), 0.1 mM dexamethasone, 0.1 mM L-ascorbic acid-2-phosphate, 10 ng/ml TGF-β1 (Peprotech)

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Manufacturer protocol

Download the product protocol from Fisher Scientific for Gibco™ DMEM, high glucose below.

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