Dulbecco’s Modified Eagle’s Medium - high glucose

Protocol tips

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	HG-DMEM supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 0.29 mg/ml L-glutamine, 1 mM sodium pyruvate, 1.25 mg/ml human serum albumin (HAS; Sigma-Aldrich), 1% ITS+1 (containing 1.0 mg/ml insulin from bovine pancreas, 0.55 mg/ml human transferrin, 0.5 μg/ml sodium selenite, 50 mg/ml bovine serum albumin and 470 μg/ml linoleic acid (Sigma-Aldrich), 0.1 μM dexamethasone, 0.1 mM L-ascorbic acid-2-phosphate, and 10 ng/ml TGF-β1 (PeproTech, Rocky Hill, NJ, USA)

Protocol tips

HG-DMEM supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 0.29 mg/ml L-glutamine, 1 mM sodium pyruvate, 1.25 mg/ml human serum albumin (HAS; Sigma-Aldrich), 1% ITS+1 (containing 1.0 mg/ml insulin from bovine pancreas, 0.55 mg/ml human transferrin, 0.5 μg/ml sodium selenite, 50 mg/ml bovine serum albumin and 470 μg/ml linoleic acid (Sigma-Aldrich), 0.1 μM dexamethasone, 0.1 mM L-ascorbic acid-2-phosphate, and 10 ng/ml TGF-β1 (PeproTech, Rocky Hill, NJ, USA)

Publication protocol

HG-DMEM supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 0.29 mg/ml L-glutamine, 1 mM sodium pyruvate, 1.25 mg/ml human serum albumin (HAS; Sigma-Aldrich), 1% ITS+1 (containing 1.0 mg/ml insulin from bovine pancreas, 0.55 mg/ml human transferrin, 0.5 μg/ml sodium selenite, 50 mg/ml bovine serum albumin and 470 μg/ml linoleic acid (Sigma-Aldrich), 0.1 μM dexamethasone, 0.1 mM L-ascorbic acid-2-phosphate, and 10 ng/ml TGF-β1 (PeproTech, Rocky Hill, NJ, USA)

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Dulbecco’s Modified Eagle’s Medium - high glucose below.

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