FlashTag™ Biotin HSR RNA Labeling Kits

Protocol tips

Upstream tips	Protocol tips	Downstream tips
		Label all samples at one time to avoid day-to-day variations. The labeled samples can be stored at -80°C upto 3 months and successive hybridizations

Downstream tips
Label all samples at one time to avoid day-to-day variations. The labeled samples can be stored at -80°C upto 3 months and successive hybridizations

Publication protocol

miRNA Array Analysis
The miRNA 2.0 Array was hybridized using 500 ng of total RNA per to standard Affymetrix protocols. The same RNA preparations used in the mRNA microarray analysis were used in miRNA array analysis. Data extraction was performed using Affymetrix Command Console software. Raw data were analyzed by the following workflow: background detection, RMA global background correlation, quantile normalization, median polish and log2-transformed with miRNA QC tool software. qPCR validation was performed as described in the Supplementary materials.



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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for FlashTag™ Biotin HSR RNA Labeling Kits below.

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