Gibco™Essential 8™ Medium

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Prior to differentiation, it is essential to ensure 80–90% of cells are undifferentiated and cultures are 70–80% confluent. Prior to dissociation cells are treated with 10 μM ROCK inhibitor (Y-27632) to reduce dissociation-induced apoptosis	E8 media supplemented with 10 μM Y-27632	Cells are then treated with 10 μM CHIR99021 supplemented E6 media—E8 media lacking the pluripotency factors FGF2 and TGF-β1—to simulate Wnt signaling during gastrulation

Upstream tips
Prior to differentiation, it is essential to ensure 80–90% of cells are undifferentiated and cultures are 70–80% confluent. Prior to dissociation cells are treated with 10 μM ROCK inhibitor (Y-27632) to reduce dissociation-induced apoptosis
Protocol tips
E8 media supplemented with 10 μM Y-27632
Downstream tips
Cells are then treated with 10 μM CHIR99021 supplemented E6 media—E8 media lacking the pluripotency factors FGF2 and TGF-β1—to simulate Wnt signaling during gastrulation

Publication protocol

E8 media supplemented with 10 μM Y-27632

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™Essential 8™ Medium below.

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