Gibco™Neurobasal™ Medium

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Colonies were treated with collagenase IV (1.5mg/mL) and mildly triturated to form a suspension of uniform clumps, transferred directly to 5mL medium	MGdM (NGD (supplementray table1) + 10ng/mL IL-34 + 10ng/mL CSF1), in ultra-low attachment 6-well plates (corning)

Upstream tips
Colonies were treated with collagenase IV (1.5mg/mL) and mildly triturated to form a suspension of uniform clumps, transferred directly to 5mL medium
Protocol tips
MGdM (NGD (supplementray table1) + 10ng/mL IL-34 + 10ng/mL CSF1), in ultra-low attachment 6-well plates (corning)

Publication protocol

MGdM (NGD (supplementray table1) + 10ng/mL IL-34 + 10ng/mL CSF1), in ultra-low attachment 6-well plates (corning)

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Reviews

Discussion

Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™Neurobasal™ Medium below.

Download manufacturer protocol

Videos

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