Microglia Medium

Protocol tips

Upstream tips	Protocol tips	Downstream tips
after mesodermal lineage differentiation	50%/50% mix of Microglia Medium (ScienCell Research, #1901) and specification medium (DMEM/F12 with 1% P/S, 1% L-glutamine, 20 ng/ml bFGF, 1 × B27, 1% FBS [Atlanta Biologicals], 10 ng/ml human granulocyte macrophage colony-stimulating factor [GM-CSF, R&D Systems, Minneapolis MN, #215-GM-050/CF], 10 ng/ml human macrophage colony-stimulating factor [M-CSF, R&D Systems, #216-MC-025], 10 ng/ml interleukin 34 [IL-34, R&D Systems, #5265-IL-010/CF], and 2 ng/ml TGFβ-1 [R&D Systems, #240-B-002])

Upstream tips
after mesodermal lineage differentiation
Protocol tips
50%/50% mix of Microglia Medium (ScienCell Research, #1901) and specification medium (DMEM/F12 with 1% P/S, 1% L-glutamine, 20 ng/ml bFGF, 1 × B27, 1% FBS [Atlanta Biologicals], 10 ng/ml human granulocyte macrophage colony-stimulating factor [GM-CSF, R&D Systems, Minneapolis MN, #215-GM-050/CF], 10 ng/ml human macrophage colony-stimulating factor [M-CSF, R&D Systems, #216-MC-025], 10 ng/ml interleukin 34 [IL-34, R&D Systems, #5265-IL-010/CF], and 2 ng/ml TGFβ-1 [R&D Systems, #240-B-002])

Publication protocol

50%/50% mix of Microglia Medium (ScienCell Research, #1901) and specification medium (DMEM/F12 with 1% P/S, 1% L-glutamine, 20 ng/ml bFGF, 1 × B27, 1% FBS [Atlanta Biologicals], 10 ng/ml human granulocyte macrophage colony-stimulating factor [GM-CSF, R&D Systems, Minneapolis MN, #215-GM-050/CF], 10 ng/ml human macrophage colony-stimulating factor [M-CSF, R&D Systems, #216-MC-025], 10 ng/ml interleukin 34 [IL-34, R&D Systems, #5265-IL-010/CF], and 2 ng/ml TGFβ-1 [R&D Systems, #240-B-002])

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Discussion

Papers

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Manufacturer protocol

Download the product protocol from ScienCell Research Laboratories for Microglia Medium below.

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Videos

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