Gibco™ RPMI 1640 Medium

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	30 ng/mL VEGF (Peprotech), 30 ng/mL BMP4 (Peprotech), 40 ng/mL SCF (Peprotech), and 50 ng/mL Activin A (Invitrogen) in RPMI medium (Life Technologies) plus 2% B27	medium was changed with RPMI plus 2% B27 supplemented with 50 ng/mL SCF, 50 ng/mL Flt3L (Peprotech), 10 ng/mL IL-3 (Peprotech), 50 ng/mL GM-CSF (Peprotech), and 25 ng/mL BMP-4

Protocol tips
30 ng/mL VEGF (Peprotech), 30 ng/mL BMP4 (Peprotech), 40 ng/mL SCF (Peprotech), and 50 ng/mL Activin A (Invitrogen) in RPMI medium (Life Technologies) plus 2% B27
Downstream tips
medium was changed with RPMI plus 2% B27 supplemented with 50 ng/mL SCF, 50 ng/mL Flt3L (Peprotech), 10 ng/mL IL-3 (Peprotech), 50 ng/mL GM-CSF (Peprotech), and 25 ng/mL BMP-4

Publication protocol

30 ng/mL VEGF (Peprotech), 30 ng/mL BMP4 (Peprotech), 40 ng/mL SCF (Peprotech), and 50 ng/mL Activin A (Invitrogen) in RPMI medium (Life Technologies) plus 2% B27

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Manufacturer protocol

Download the product protocol from Fisher Scientific for Gibco™ RPMI 1640 Medium below.

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