Gibco™ MEM α, Nucleosides

Protocol tips

Upstream tips	Protocol tips	Downstream tips
with or without TH (Takeda Chemical Industries (Osaka, Japan), was dissolved in DMSO and added to OM or regular medium (RM) (Dulbecco’s modified Eagle’s medium (DMEM; Gibco/BRL) supplemented with 10% FBS and 1% P/S))	αMEM containing 10% FBS, 1% P/S, 10 nM dexamethasone (Dex; Wako Pure Chemical Industries), 10 mM β-glycerophosphate (β-GP; Sigma-Aldrich), and 100 μM l-ascorbate-2-phosphate (AsAp; Wako Pure Chemical Industries)

Upstream tips
with or without TH (Takeda Chemical Industries (Osaka, Japan), was dissolved in DMSO and added to OM or regular medium (RM) (Dulbecco’s modified Eagle’s medium (DMEM; Gibco/BRL) supplemented with 10% FBS and 1% P/S))
Protocol tips
αMEM containing 10% FBS, 1% P/S, 10 nM dexamethasone (Dex; Wako Pure Chemical Industries), 10 mM β-glycerophosphate (β-GP; Sigma-Aldrich), and 100 μM l-ascorbate-2-phosphate (AsAp; Wako Pure Chemical Industries)

Publication protocol

αMEM containing 10% FBS, 1% P/S, 10 nM dexamethasone (Dex; Wako Pure Chemical Industries), 10 mM β-glycerophosphate (β-GP; Sigma-Aldrich), and 100 μM l-ascorbate-2-phosphate (AsAp; Wako Pure Chemical Industries)

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Manufacturer protocol

Download the product protocol from Fisher Scientific for Gibco™ MEM α, Nucleosides below.

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