Minimum Essential Medium Eagle

Protocol tips

Upstream tips	Protocol tips	Downstream tips
supplemented with 100 μM 2P-ascorbic acid, 100 nM dexamethasone, and 10 mM b-glycerophosphate	α-MEM with 10% heat-inactivated foetal bovine serum (FBS), 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin)

Upstream tips
supplemented with 100 μM 2P-ascorbic acid, 100 nM dexamethasone, and 10 mM b-glycerophosphate
Protocol tips
α-MEM with 10% heat-inactivated foetal bovine serum (FBS), 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin)

Publication protocol

α-MEM with 10% heat-inactivated foetal bovine serum (FBS), 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin)

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for Minimum Essential Medium Eagle below.

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