HyClone Minimal Essential Medium (MEM) variations: Liquid

Protocol tips

Upstream tips	Protocol tips	Downstream tips
medium supplemented with 10 μg/ml ITS-X (Invitrogen), 5.35 μg/ml linoleic acid, 1.25 μg/ml bovine serum albumin, 1.0 μg/ml dexamethasone and 10 ng/ml TGF-β3 (Chemicon, Temecula, CA, USA)	α-Minimal Essential Medium (MEM) (Hyclone, Logan, UT, USA), 20% fetal bovine serum (Hyclone), 100 μM L-ascorbic acid-2-phosphate (Sigma, St Louis, MO, USA), 100 units/ml penicillin (Sigma) and 100 μg/ml streptomycin (Invitrogen)

Upstream tips
medium supplemented with 10 μg/ml ITS-X (Invitrogen), 5.35 μg/ml linoleic acid, 1.25 μg/ml bovine serum albumin, 1.0 μg/ml dexamethasone and 10 ng/ml TGF-β3 (Chemicon, Temecula, CA, USA)
Protocol tips
α-Minimal Essential Medium (MEM) (Hyclone, Logan, UT, USA), 20% fetal bovine serum (Hyclone), 100 μM L-ascorbic acid-2-phosphate (Sigma, St Louis, MO, USA), 100 units/ml penicillin (Sigma) and 100 μg/ml streptomycin (Invitrogen)

Publication protocol

α-Minimal Essential Medium (MEM) (Hyclone, Logan, UT, USA), 20% fetal bovine serum (Hyclone), 100 μM L-ascorbic acid-2-phosphate (Sigma, St Louis, MO, USA), 100 units/ml penicillin (Sigma) and 100 μg/ml streptomycin (Invitrogen)

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Manufacturer protocol

Download the product protocol from Cytiva for HyClone Minimal Essential Medium (MEM) variations: Liquid below.

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