Gibco™DMEM/F-12

Stem cell culture media Rat oligodendrocyte precursors (OPCs)

Experiment
Stem cell culture media Rat oligodendrocyte precursors (OPCs)
Product
Gibco™DMEM/F-12 from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
mNSCs or rNSCs were dissociated to a single cell suspension using Accutase (Gibco). The cells were counted and plated at 4 × 104 cells/cm2 on plates, which were coated with 0.1 mg/mL poly-L-ornithine (Sigma) and then with 10 µg/mL laminin (Sigma), or coated only with 5% Matrigel (BD Biosciences)
Protocol tips
DMEM/F12, Gibco) supplemented with 1× B27 (Gibco), 1× N2 (Gibco), 20 ng/mL FGF2 (Pepro Tech), 20 ng/mL PDGF-AA (Pepro Tech), and 0.4 µM SAG (Enzo Life Sciences) or 200 ng/mL SHH (R&D Systems)

Publication protocol

DMEM/F12, Gibco) supplemented with 1× B27 (Gibco), 1× N2 (Gibco), 20 ng/mL FGF2 (Pepro Tech), 20 ng/mL PDGF-AA (Pepro Tech), and 0.4 µM SAG (Enzo Life Sciences) or 200 ng/mL SHH (R&D Systems)

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™DMEM/F-12 below.

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