mTeSR™1

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Cells were cultured on matrigel-coated plates (Corning 354,277) in mTeSR1 medium (Stem Cell Technologies 05850), and maintained at 37 °C under conditions of 5% CO2.	Cells were passaged every 4 or 5 days using 0.5 mM EDTA (Table 1).

Protocol tips
Cells were cultured on matrigel-coated plates (Corning 354,277) in mTeSR1 medium (Stem Cell Technologies 05850), and maintained at 37 °C under conditions of 5% CO2.
Downstream tips
Cells were passaged every 4 or 5 days using 0.5 mM EDTA (Table 1).

Publication protocol

Cells were cultured on matrigel-coated plates (Corning 354,277) in mTeSR1 medium (Stem Cell Technologies 05850), and maintained at 37 °C under conditions of 5% CO2.

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