Protocol tips
Upstream tips |
Protocol tips |
Downstream tips |
The hESC lines were maintained on an inactivated mouse embryonic fibroblast (MEF) |
Dulbecco's Modified Eagle's Medium/Ham's F12 (DMEM/F12) supplemented with KSR 20% 2 mM non-essential amino acids, 2 mM L-glutamine, 100 U/ml penicillin, 50 μg/ml streptomycin, 0.1 mM β-mercaptoethanol and 4 ng/ml of bFGF |
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Upstream tips |
The hESC lines were maintained on an inactivated mouse embryonic fibroblast (MEF) |
Protocol tips |
Dulbecco's Modified Eagle's Medium/Ham's F12 (DMEM/F12) supplemented with KSR 20% 2 mM non-essential amino acids, 2 mM L-glutamine, 100 U/ml penicillin, 50 μg/ml streptomycin, 0.1 mM β-mercaptoethanol and 4 ng/ml of bFGF |
Publication protocol
Dulbecco's Modified Eagle's Medium/Ham's F12 (DMEM/F12) supplemented with KSR 20% 2 mM non-essential amino acids, 2 mM L-glutamine, 100 U/ml penicillin, 50 μg/ml streptomycin, 0.1 mM β-mercaptoethanol and 4 ng/ml of bFGF
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