Gibco™Neurobasal™-A Medium

Stem cell culture media GBM patient-derived stem cells

5/5 - 1 Review - 100% recommends this

Experiment

Stem cell culture media GBM patient-derived stem cells

Product

Gibco™Neurobasal™-A Medium from Thermo Fisher Scientific

Manufacturer

Thermo Fisher Scientific

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Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Neurobasal A-Medium (Gibco Life Technologies, Bleiswijk, The Netherlands) supplemented with 2% B27 supplement (Gibco Life Technologies), 20ng/ml EGF (R&D systems, Abingdon, UK), 20 ng/ml bFGF (Merck-Millipore, Billerica, MA, USA), 1% pen/strep and 1% L-glutamine (Gibco Life Technologies)

Protocol tips
Neurobasal A-Medium (Gibco Life Technologies, Bleiswijk, The Netherlands) supplemented with 2% B27 supplement (Gibco Life Technologies), 20ng/ml EGF (R&D systems, Abingdon, UK), 20 ng/ml bFGF (Merck-Millipore, Billerica, MA, USA), 1% pen/strep and 1% L-glutamine (Gibco Life Technologies)

Publication protocol

Neurobasal A-Medium (Gibco Life Technologies, Bleiswijk, The Netherlands) supplemented with 2% B27 supplement (Gibco Life Technologies), 20ng/ml EGF (R&D systems, Abingdon, UK), 20 ng/ml bFGF (Merck-Millipore, Billerica, MA, USA), 1% pen/strep and 1% L-glutamine (Gibco Life Technologies)

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Reviews

Scientist

Review date

2021-05-17

Protocol

Adjusted

Changes to upstream processing

supplemented with 2% B27 supplement (Gibco Life Technologies), 20ng/ml EGF (R&D systems, Abingdon, UK), 20 ng/ml bFGF (Merck-Millipore, Billerica, MA, USA), 1% pen/strep and 1% L-glutamine (Gibco Life Technologies)

Protocol tips

Check out every 2 to 3 days acidity of medium or size of neurospeheres

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™Neurobasal™-A Medium below.

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