Gibco™ DMEM/F-12, GlutaMAX™ supplement

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Matrigel (Corning 356,230) or fibronectin-coated 6-well plates in	stem-cell growth medium (GM) containing DMEM/F-12 plus Glutamax (Life Technologies 10,565–018), BIT9500 serum substitute (1×, Stem Cell Technologies 09500), heparin (20 μg/mL Sigma H3149), primocin (1×, InvivoGen ant-pm-1), human bFGF (20 ng/mL, Stemgent 03–0002), and human EGF (20 ng/mL, R&D Biosystems 236-EG)

Upstream tips
Matrigel (Corning 356,230) or fibronectin-coated 6-well plates in
Protocol tips
stem-cell growth medium (GM) containing DMEM/F-12 plus Glutamax (Life Technologies 10,565–018), BIT9500 serum substitute (1×, Stem Cell Technologies 09500), heparin (20 μg/mL Sigma H3149), primocin (1×, InvivoGen ant-pm-1), human bFGF (20 ng/mL, Stemgent 03–0002), and human EGF (20 ng/mL, R&D Biosystems 236-EG)

Publication protocol

stem-cell growth medium (GM) containing DMEM/F-12 plus Glutamax (Life Technologies 10,565–018), BIT9500 serum substitute (1×, Stem Cell Technologies 09500), heparin (20 μg/mL Sigma H3149), primocin (1×, InvivoGen ant-pm-1), human bFGF (20 ng/mL, Stemgent 03–0002), and human EGF (20 ng/mL, R&D Biosystems 236-EG)

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™ DMEM/F-12, GlutaMAX™ supplement below.

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