Gibco™DMEM/F-12

Protocol tips

Upstream tips	Protocol tips	Downstream tips
used for hESC maintenance only with MEFs	DMEM/F12 (medium AD, Invitrogen) supplemented with 0.1% (w/v) BSA (Sigma), 2mM L-glutamine and 1% NEAA, 0.1mM β-mercaptoethanol, 1x lipid supplement, 1x N2 supplement,1x B27 supplement, 20ng/ml bFGF, 10ng/ml Activin A (R&D) and 2ng/ml NT4 (Preprotech).

Upstream tips
used for hESC maintenance only with MEFs
Protocol tips
DMEM/F12 (medium AD, Invitrogen) supplemented with 0.1% (w/v) BSA (Sigma), 2mM L-glutamine and 1% NEAA, 0.1mM β-mercaptoethanol, 1x lipid supplement, 1x N2 supplement,1x B27 supplement, 20ng/ml bFGF, 10ng/ml Activin A (R&D) and 2ng/ml NT4 (Preprotech).

Publication protocol

DMEM/F12 (medium AD, Invitrogen) supplemented with 0.1% (w/v) BSA (Sigma), 2mM L-glutamine and 1% NEAA, 0.1mM β-mercaptoethanol, 1x lipid supplement, 1x N2 supplement,1x B27 supplement, 20ng/ml bFGF, 10ng/ml Activin A (R&D) and 2ng/ml NT4 (Preprotech).

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Stem cell culture media hESC standard maintenance with MEFs using Gibco™DMEM/F-12 from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™DMEM/F-12 below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Stem cell culture media hESC standard maintenance with MEFs using Gibco™DMEM/F-12 from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.