Gibco™KnockOut™ DMEM

Protocol tips

Upstream tips	Protocol tips	Downstream tips
hESC lines on mouse embryonic fibroblasts (MEFs)	DMEM (KO-DMEM, Invitrogen, Paisley, UK) supplemented with 20% (v/v) knockout serum replacement (KO-SR, Invitrogen), 10 ng/ml basic fibroblast growth factor (bFGF, Autogen Bioclear), 2 mM L-glutamine, 1% non-essential amino acids (NEAA) and 0.1 mM β-mercaptoethanol and 1% penicillin-streptomycin (Life Tech, Paisley, UK)

Upstream tips
hESC lines on mouse embryonic fibroblasts (MEFs)
Protocol tips
DMEM (KO-DMEM, Invitrogen, Paisley, UK) supplemented with 20% (v/v) knockout serum replacement (KO-SR, Invitrogen), 10 ng/ml basic fibroblast growth factor (bFGF, Autogen Bioclear), 2 mM L-glutamine, 1% non-essential amino acids (NEAA) and 0.1 mM β-mercaptoethanol and 1% penicillin-streptomycin (Life Tech, Paisley, UK)

Publication protocol

DMEM (KO-DMEM, Invitrogen, Paisley, UK) supplemented with 20% (v/v) knockout serum replacement (KO-SR, Invitrogen), 10 ng/ml basic fibroblast growth factor (bFGF, Autogen Bioclear), 2 mM L-glutamine, 1% non-essential amino acids (NEAA) and 0.1 mM β-mercaptoethanol and 1% penicillin-streptomycin (Life Tech, Paisley, UK)

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™KnockOut™ DMEM below.

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