EmbryoMax MEF Conditioned Media

Protocol tips

Upstream tips	Protocol tips	Downstream tips
cultured on BD Matrigel (BD Biosciences) matrix-coated tissue culture plates to 70% confluence	mouse embryonic fibroblast–conditioned medium supplemented with 5 ng/mL basic fibroblast growth factor (bFGF, Sigma)

Upstream tips
cultured on BD Matrigel (BD Biosciences) matrix-coated tissue culture plates to 70% confluence
Protocol tips
mouse embryonic fibroblast–conditioned medium supplemented with 5 ng/mL basic fibroblast growth factor (bFGF, Sigma)

Publication protocol

mouse embryonic fibroblast–conditioned medium supplemented with 5 ng/mL basic fibroblast growth factor (bFGF, Sigma)

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for EmbryoMax MEF Conditioned Media below.

Download manufacturer protocol

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