EmbryoMax MEF Conditioned Media

Protocol tips

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	70% (v/v) mouse embryonic fibroblast-conditioned medium (MEF-CM) and 30% TS medium (RPMI 1640 supplemented with 20% FBS (Invitrogen), 0.5% penicillin-streptomycin (Thermo Fisher Scientific), 1% GlutaMAX (Thermo Fisher Scientific), 1% sodium pyruvate (Thermo Fisher Scientific) and 0.1 mM 2-mercaptoethanol (Thermo Fisher Scientific) that was supplemented with 20 μM Y-27632 (MCE), 37.5 ng/ml FGF4 (Sigma-Aldrich), and 1.5 μg/ml Heparin (Sigma-Aldrich)

Protocol tips

70% (v/v) mouse embryonic fibroblast-conditioned medium (MEF-CM) and 30% TS medium (RPMI 1640 supplemented with 20% FBS (Invitrogen), 0.5% penicillin-streptomycin (Thermo Fisher Scientific), 1% GlutaMAX (Thermo Fisher Scientific), 1% sodium pyruvate (Thermo Fisher Scientific) and 0.1 mM 2-mercaptoethanol (Thermo Fisher Scientific) that was supplemented with 20 μM Y-27632 (MCE), 37.5 ng/ml FGF4 (Sigma-Aldrich), and 1.5 μg/ml Heparin (Sigma-Aldrich)

Publication protocol

70% (v/v) mouse embryonic fibroblast-conditioned medium (MEF-CM) and 30% TS medium (RPMI 1640 supplemented with 20% FBS (Invitrogen), 0.5% penicillin-streptomycin (Thermo Fisher Scientific), 1% GlutaMAX (Thermo Fisher Scientific), 1% sodium pyruvate (Thermo Fisher Scientific) and 0.1 mM 2-mercaptoethanol (Thermo Fisher Scientific) that was supplemented with 20 μM Y-27632 (MCE), 37.5 ng/ml FGF4 (Sigma-Aldrich), and 1.5 μg/ml Heparin (Sigma-Aldrich)

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for EmbryoMax MEF Conditioned Media below.

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