Gibco™ DMEM/F-12, GlutaMAX™ supplement

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	DMEM/F-12, GlutaMAX(TM) (Gibco) medium supplemented with 1% N-2 supplement (Invitrogen), 1% Chemically Defined Lipid Concentrate (Invitrogen), 0.25 mg ml−1 fungizone (Gibco), 100 U ml−1 penicillin and 100 μg ml−1 streptomycin under 40% O2/5% CO2 conditions.

Protocol tips
DMEM/F-12, GlutaMAX(TM) (Gibco) medium supplemented with 1% N-2 supplement (Invitrogen), 1% Chemically Defined Lipid Concentrate (Invitrogen), 0.25 mg ml−1 fungizone (Gibco), 100 U ml−1 penicillin and 100 μg ml−1 streptomycin under 40% O2/5% CO2 conditions.

Publication protocol

DMEM/F-12, GlutaMAX(TM) (Gibco) medium supplemented with 1% N-2 supplement (Invitrogen), 1% Chemically Defined Lipid Concentrate (Invitrogen), 0.25 mg ml−1 fungizone (Gibco), 100 U ml−1 penicillin and 100 μg ml−1 streptomycin under 40% O2/5% CO2 conditions.

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™ DMEM/F-12, GlutaMAX™ supplement below.

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