DMEM (Dulbecco's Modified Eagle's medium)

Protocol tips

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	Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 20% knockout serum replacement, 1 mM l-glutamine, 0.1 mM β-mercaptoethanol, 1% nonessential amino acid stock (all from life technologies, Darmstadt, Germany), and 10 ng/ml basic fibroblast growth factor (bFGF; supplied by the Institute for Technical Chemistry, Leibniz University, Hannover, Germany)

Protocol tips
Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 20% knockout serum replacement, 1 mM l-glutamine, 0.1 mM β-mercaptoethanol, 1% nonessential amino acid stock (all from life technologies, Darmstadt, Germany), and 10 ng/ml basic fibroblast growth factor (bFGF; supplied by the Institute for Technical Chemistry, Leibniz University, Hannover, Germany)

Publication protocol

Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 20% knockout serum replacement, 1 mM l-glutamine, 0.1 mM β-mercaptoethanol, 1% nonessential amino acid stock (all from life technologies, Darmstadt, Germany), and 10 ng/ml basic fibroblast growth factor (bFGF; supplied by the Institute for Technical Chemistry, Leibniz University, Hannover, Germany)

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Manufacturer protocol

Download the product protocol from Sigma-Aldrich for DMEM (Dulbecco's Modified Eagle's medium) below.

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