Gibco DMEM/F-12, HEPES

Protocol tips

Upstream tips	Protocol tips	Downstream tips
25,000 cells/ml in 2ml/well	DMEM/Ham’s F-12 (1:1) with 15mM HEPES supplemented with B27 (Invitrogen), 20 ng/ml EGF (Invitrogen), 20 ng/ml bFGF (Invitrogen), antibiotic-antimycotic (100 unit/ml penicillin G sodium, 100 ug/ml streptomycin sulfate and 0.25 ug/ml amphotericin B) (Invitrogen), 2 μg/ml Heparin (Stem Cell Technologies, 0.5 μg/ml Hydrocortisone (Stem Cell Technologies) and 5 ug/ml Insulin (Sigma)

Upstream tips
25,000 cells/ml in 2ml/well
Protocol tips
DMEM/Ham’s F-12 (1:1) with 15mM HEPES supplemented with B27 (Invitrogen), 20 ng/ml EGF (Invitrogen), 20 ng/ml bFGF (Invitrogen), antibiotic-antimycotic (100 unit/ml penicillin G sodium, 100 ug/ml streptomycin sulfate and 0.25 ug/ml amphotericin B) (Invitrogen), 2 μg/ml Heparin (Stem Cell Technologies, 0.5 μg/ml Hydrocortisone (Stem Cell Technologies) and 5 ug/ml Insulin (Sigma)

Publication protocol

DMEM/Ham’s F-12 (1:1) with 15mM HEPES supplemented with B27 (Invitrogen), 20 ng/ml EGF (Invitrogen), 20 ng/ml bFGF (Invitrogen), antibiotic-antimycotic (100 unit/ml penicillin G sodium, 100 ug/ml streptomycin sulfate and 0.25 ug/ml amphotericin B) (Invitrogen), 2 μg/ml Heparin (Stem Cell Technologies, 0.5 μg/ml Hydrocortisone (Stem Cell Technologies) and 5 ug/ml Insulin (Sigma)

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Stem cell culture media hMammospheres using Gibco DMEM/F-12, HEPES from Thermo Fisher Scientific.

Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco DMEM/F-12, HEPES below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Stem cell culture media hMammospheres using Gibco DMEM/F-12, HEPES from Thermo Fisher Scientific. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.