Gibco DMEM/F-12, HEPES

Stem cell culture media hMammospheres

Experiment
Stem cell culture media hMammospheres
Product
Gibco DMEM/F-12, HEPES from Thermo Fisher Scientific
Manufacturer
Thermo Fisher Scientific

Protocol tips

Upstream tips
25,000 cells/ml in 2ml/well
Protocol tips
DMEM/Ham’s F-12 (1:1) with 15mM HEPES supplemented with B27 (Invitrogen), 20 ng/ml EGF (Invitrogen), 20 ng/ml bFGF (Invitrogen), antibiotic-antimycotic (100 unit/ml penicillin G sodium, 100 ug/ml streptomycin sulfate and 0.25 ug/ml amphotericin B) (Invitrogen), 2 μg/ml Heparin (Stem Cell Technologies, 0.5 μg/ml Hydrocortisone (Stem Cell Technologies) and 5 ug/ml Insulin (Sigma)

Publication protocol

DMEM/Ham’s F-12 (1:1) with 15mM HEPES supplemented with B27 (Invitrogen), 20 ng/ml EGF (Invitrogen), 20 ng/ml bFGF (Invitrogen), antibiotic-antimycotic (100 unit/ml penicillin G sodium, 100 ug/ml streptomycin sulfate and 0.25 ug/ml amphotericin B) (Invitrogen), 2 μg/ml Heparin (Stem Cell Technologies, 0.5 μg/ml Hydrocortisone (Stem Cell Technologies) and 5 ug/ml Insulin (Sigma)

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Papers

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco DMEM/F-12, HEPES below.

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