Gibco™ RPMI 1640 Medium

Protocol tips

Upstream tips	Protocol tips	Downstream tips
All cells were cultured in RPMI 1640 in ultralow attachment cell culture flasks were used	serum-free medium supplemented with 20 ng/mL epidermal growth factor (EGF) and 10 ng/mL basic fibroblast growth factor (bFGF)

Upstream tips
All cells were cultured in RPMI 1640 in ultralow attachment cell culture flasks were used
Protocol tips
serum-free medium supplemented with 20 ng/mL epidermal growth factor (EGF) and 10 ng/mL basic fibroblast growth factor (bFGF)

Publication protocol

serum-free medium supplemented with 20 ng/mL epidermal growth factor (EGF) and 10 ng/mL basic fibroblast growth factor (bFGF)

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Manufacturer protocol

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