IntestiCult™ Organoid Growth Medium (Mouse)

Protocol tips

Upstream tips	Protocol tips	Downstream tips
Pelleted crypt structures were resuspended in a 1:1 mixture of Intesticult medium (Stemcell technologies, Köln, Germany) and growth factor reduced matrigel (Corning, NY, USA)	Intesticult medium supplemented with 100 U/ml Penicillin, 100 μg/ml Streptomycin, 2.5 μg/ml amphotericin B, 2.05 mg/ml sodium deoxycholate (all Thermo Fisher scientific) and 20 μg/ml Gentamycin (Lonza, Basel Switzerland) was added (300 μl/well)

Upstream tips
Pelleted crypt structures were resuspended in a 1:1 mixture of Intesticult medium (Stemcell technologies, Köln, Germany) and growth factor reduced matrigel (Corning, NY, USA)
Protocol tips
Intesticult medium supplemented with 100 U/ml Penicillin, 100 μg/ml Streptomycin, 2.5 μg/ml amphotericin B, 2.05 mg/ml sodium deoxycholate (all Thermo Fisher scientific) and 20 μg/ml Gentamycin (Lonza, Basel Switzerland) was added (300 μl/well)

Publication protocol

Intesticult medium supplemented with 100 U/ml Penicillin, 100 μg/ml Streptomycin, 2.5 μg/ml amphotericin B, 2.05 mg/ml sodium deoxycholate (all Thermo Fisher scientific) and 20 μg/ml Gentamycin (Lonza, Basel Switzerland) was added (300 μl/well)

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Manufacturer protocol

Download the product protocol from STEMCELL technologies for IntestiCult™ Organoid Growth Medium (Mouse) below.

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