Gibco™Advanced DMEM/F-12

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Advanced DMEM/F12 supplemented with 10 × 10−3 m HEPES, 1x Glutamax, 1% Penicillin/Streptomycin, 1x B27, 1.25 × 10−3 m N ‐acetylcysteine, 50% Wnt3a CM (produced in‐house), 20% R‐spondin‐1 CM, 10% noggin CM, 10 × 10−3 m nicotinamide (Sigma), 50 ng mL−1 human EGF, 10 × 10−9 m Gastrin (Tocris Bioscience), 0.5 × 10−6 m A83‐01 (Tocris Bioscience) and 10 × 10−6 m SB202190 (Sigma)

Protocol tips
Advanced DMEM/F12 supplemented with 10 × 10−3 m HEPES, 1x Glutamax, 1% Penicillin/Streptomycin, 1x B27, 1.25 × 10−3 m N ‐acetylcysteine, 50% Wnt3a CM (produced in‐house), 20% R‐spondin‐1 CM, 10% noggin CM, 10 × 10−3 m nicotinamide (Sigma), 50 ng mL−1 human EGF, 10 × 10−9 m Gastrin (Tocris Bioscience), 0.5 × 10−6 m A83‐01 (Tocris Bioscience) and 10 × 10−6 m SB202190 (Sigma)

Publication protocol

Advanced DMEM/F12 supplemented with 10 × 10−3 m HEPES, 1x Glutamax, 1% Penicillin/Streptomycin, 1x B27, 1.25 × 10−3 m N ‐acetylcysteine, 50% Wnt3a CM (produced in‐house), 20% R‐spondin‐1 CM, 10% noggin CM, 10 × 10−3 m nicotinamide (Sigma), 50 ng mL−1 human EGF, 10 × 10−9 m Gastrin (Tocris Bioscience), 0.5 × 10−6 m A83‐01 (Tocris Bioscience) and 10 × 10−6 m SB202190 (Sigma)

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™Advanced DMEM/F-12 below.

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