StemSpan™ SFEM

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	StemSpan SFEM media (Stem Cell Technologies), L-glutamine (100ng/mL), penicillin/streptomycin (100ng/mL) and the following human cytokines: SCF (20ng/mL, Peprotech), Flt3-L (20ng/mL, Peprotech), TPO (50ng/mL, Peprotech), IL-3 (20ng/mL, Peprotech), IL-6 (20ng/mL, Peprotech), G-CSF (20ng/mL, Peprotech), IL-5 (20ng/mL, Peprotech), M-CSF (20ng/mL, Peprotech), GM-CSF (20ng/mL, Peprotech) and EPO (4U/mL, R&D)

Protocol tips

StemSpan SFEM media (Stem Cell Technologies), L-glutamine (100ng/mL), penicillin/streptomycin (100ng/mL) and the following human cytokines: SCF (20ng/mL, Peprotech), Flt3-L (20ng/mL, Peprotech), TPO (50ng/mL, Peprotech), IL-3 (20ng/mL, Peprotech), IL-6 (20ng/mL, Peprotech), G-CSF (20ng/mL, Peprotech), IL-5 (20ng/mL, Peprotech), M-CSF (20ng/mL, Peprotech), GM-CSF (20ng/mL, Peprotech) and EPO (4U/mL, R&D)

Publication protocol

StemSpan SFEM media (Stem Cell Technologies), L-glutamine (100ng/mL), penicillin/streptomycin (100ng/mL) and the following human cytokines: SCF (20ng/mL, Peprotech), Flt3-L (20ng/mL, Peprotech), TPO (50ng/mL, Peprotech), IL-3 (20ng/mL, Peprotech), IL-6 (20ng/mL, Peprotech), G-CSF (20ng/mL, Peprotech), IL-5 (20ng/mL, Peprotech), M-CSF (20ng/mL, Peprotech), GM-CSF (20ng/mL, Peprotech) and EPO (4U/mL, R&D)

Full paper   Login or join for free to view the full paper.

Reviews

Discussion

Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Stem cell culture media hHSCs using StemSpan™ SFEM from STEMCELL technologies.

Manufacturer protocol

Download the product protocol from STEMCELL technologies for StemSpan™ SFEM below.

Download manufacturer protocol

Videos

Check out videos that might be relevant for performing Stem cell culture media hHSCs using StemSpan™ SFEM from STEMCELL technologies. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.