Gibco™ RPMI 1640 Medium

Protocol tips

Upstream tips	Protocol tips	Downstream tips
CD34+ Cells	RPMI-1640 medium (Lonza, 12-702Q) with 10% fetal bovine serum (GE Healthcare HyClone. SH30071.03), 100 ng/mL stem cell factor (SCF) (R&D Systems, #7466-SC-010/CF), thrombopoietin (TPO) (R&D Systems, #288-TP-200/CF), Fms-like tyrosine kinase 3 ligand (FL) (BioLegend, # 710802)

Upstream tips
CD34+ Cells
Protocol tips
RPMI-1640 medium (Lonza, 12-702Q) with 10% fetal bovine serum (GE Healthcare HyClone. SH30071.03), 100 ng/mL stem cell factor (SCF) (R&D Systems, #7466-SC-010/CF), thrombopoietin (TPO) (R&D Systems, #288-TP-200/CF), Fms-like tyrosine kinase 3 ligand (FL) (BioLegend, # 710802)

Publication protocol

RPMI-1640 medium (Lonza, 12-702Q) with 10% fetal bovine serum (GE Healthcare HyClone. SH30071.03), 100 ng/mL stem cell factor (SCF) (R&D Systems, #7466-SC-010/CF), thrombopoietin (TPO) (R&D Systems, #288-TP-200/CF), Fms-like tyrosine kinase 3 ligand (FL) (BioLegend, # 710802)

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Manufacturer protocol

Download the product protocol from Fisher Scientific for Gibco™ RPMI 1640 Medium below.

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