Gibco™DMEM/F-12

Protocol tips

Upstream tips	Protocol tips	Downstream tips
	Dulbecco's modified Eagle's medium:F12 medium containing Pen/Strep antibiotics (Invitrogen), Glutamax (Invitrogen), 20 ng/mL epidermal growth factor (EGF) (Sigma Aldrich, https://www.sigmaaldrich.com/), 20 ng mL fibroblast growth factor‐2 (Sigma), N2 (Invitrogen), 10 µg/mL insulin (Sigma), and 1 µM dexamethasone (Sigma)

Protocol tips
Dulbecco's modified Eagle's medium:F12 medium containing Pen/Strep antibiotics (Invitrogen), Glutamax (Invitrogen), 20 ng/mL epidermal growth factor (EGF) (Sigma Aldrich, https://www.sigmaaldrich.com/), 20 ng mL fibroblast growth factor‐2 (Sigma), N2 (Invitrogen), 10 µg/mL insulin (Sigma), and 1 µM dexamethasone (Sigma)

Publication protocol

Dulbecco's modified Eagle's medium:F12 medium containing Pen/Strep antibiotics (Invitrogen), Glutamax (Invitrogen), 20 ng/mL epidermal growth factor (EGF) (Sigma Aldrich, https://www.sigmaaldrich.com/), 20 ng mL fibroblast growth factor‐2 (Sigma), N2 (Invitrogen), 10 µg/mL insulin (Sigma), and 1 µM dexamethasone (Sigma)

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Manufacturer protocol

Download the product protocol from Thermo Fisher Scientific for Gibco™DMEM/F-12 below.

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