DMEM/F12 - Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12

Protocol tips

Upstream tips	Protocol tips	Downstream tips
5 × 104 cells per well, in ultra-low attachment 6-well culture plates	Dulbecco’s modified Eagle’s medium (DMEM)/F12 (BI, Kibbutz Beit-Haemek, Israel), 10 ng/mL basic fibroblast growth factor, 20 ng/mL epidermal growth factor (PeproTech, Rocky Hill, USA), 1 mg/mL insulin (Sigma-Aldrich, Burlington, MA, USA), 10 μL/mL B27 additive (Life Technologies, Carlsbad, California, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin

Upstream tips
5 × 104 cells per well, in ultra-low attachment 6-well culture plates
Protocol tips
Dulbecco’s modified Eagle’s medium (DMEM)/F12 (BI, Kibbutz Beit-Haemek, Israel), 10 ng/mL basic fibroblast growth factor, 20 ng/mL epidermal growth factor (PeproTech, Rocky Hill, USA), 1 mg/mL insulin (Sigma-Aldrich, Burlington, MA, USA), 10 μL/mL B27 additive (Life Technologies, Carlsbad, California, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin

Publication protocol

Dulbecco’s modified Eagle’s medium (DMEM)/F12 (BI, Kibbutz Beit-Haemek, Israel), 10 ng/mL basic fibroblast growth factor, 20 ng/mL epidermal growth factor (PeproTech, Rocky Hill, USA), 1 mg/mL insulin (Sigma-Aldrich, Burlington, MA, USA), 10 μL/mL B27 additive (Life Technologies, Carlsbad, California, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing Stem cell culture media Ovarian cancer stem cells (Caov3, 3AO, SKOV3) using DMEM/F12 - Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 from Biological Industries.

Manufacturer protocol

Download the product protocol from Biological Industries for DMEM/F12 - Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 below.

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Videos

Check out videos that might be relevant for performing Stem cell culture media Ovarian cancer stem cells (Caov3, 3AO, SKOV3) using DMEM/F12 - Dulbecco's Modified Eagle Medium: Nutrient Mixture F-12 from Biological Industries. Please note that these videos are representative and steps or experiment specific processes must be kept in mind to expect desired results.