Corning® 500 mL SF Medium, [+] L-glutamine and 1 g/L BSA

Stem cell culture media Ovarian cancer stem cells (Caov3, 3AO, SKOV3)

Experiment
Stem cell culture media Ovarian cancer stem cells (Caov3, 3AO, SKOV3)
Product
Corning® 500 mL SF Medium, [+] L-glutamine and 1 g/L BSA from Corning
Manufacturer
Corning

Protocol tips

Upstream tips
density of 50,000 cells/ml
Protocol tips
serum-free DMEM/F12 medium (Cellgro, Virginia, USA) composed of 10 ng/ml basic fibroblast growth factor (bFGF, Peprotech, Rocky Hill, NJ, USA) and 20 ng/ml epidermal growth factor (EGF, Peprotech), 1 mg/ML insulin (Sigma–Aldrich, St Louis, MO, USA), and 1 × B27 supplement (Life Technologies,Carlsbad, CA, USA) on the ultralow attachplates (Corning, Steuben County, NY, USA)

Publication protocol

serum-free DMEM/F12 medium (Cellgro, Virginia, USA) composed of 10 ng/ml basic fibroblast growth factor (bFGF, Peprotech, Rocky Hill, NJ, USA) and 20 ng/ml epidermal growth factor (EGF, Peprotech), 1 mg/ML insulin (Sigma–Aldrich, St Louis, MO, USA), and 1 × B27 supplement (Life Technologies,Carlsbad, CA, USA) on the ultralow attachplates (Corning, Steuben County, NY, USA)

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Papers

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Manufacturer protocol

Download the product protocol from Corning for Corning® 500 mL SF Medium, [+] L-glutamine and 1 g/L BSA below.

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